![]() We performed intracellular recordings of mouse spinal motoneurons and recorded their firing in response to pulses and ramps of current in Bridge and DCC mode at various switching rates. ![]() Here, we show that suboptimal switching rates lead to an overestimation of cell excitability. ![]() It is well known that a poor choice of DCC switching rate can lead to underestimation or overestimation of the cell potential however, its effect on the cell firing is rarely discussed. Intracellular recordings using sharp microelectrodes often rely on a technique called discontinuous current-clamp (DCC) to accurately record the membrane potential while injecting current through the same microelectrode.
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